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Die Burg Hardeg in Hardegsen
News und Infos

HP Hands-on-IVF-Bericht

Hands-on-Methoden Programm

Fotoprotokoll Patientin-Labor

Learning Schedule Overview

In-vitro-Maturation porciner Oozyten auf Feederlayer-Kulturen mit Antikörpern gegen Inhibin

Identifikation und funktionale Charakterisierung von Duftrezeptorproteinen in Säugetierkeimzellen

Vitrifikation von Mäuseblastocysten mit der OPS-Methode unter Zusatz verschiedener Saccharosekonzentrationen

Einfluss von Anti-Inhibin und Kokultursystemen auf die In-vitro-Maturation und Befruchtung von Schweineeizellen

Überlebensfähigkeit von kryokonservierten Mäuseembryonen

IVF: Hoffnung für Paare mit Kinderwunsch

Ashgabat Medical Center introduces embryo implantation


23.04.2009

Basic comments on the potential of stem cell therapy in veterinary medicine and first results of mesenchymal stem cell culture

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After a long planning phase, finally our first course in "Hands-on methods in the IVF laboratory" was reality and took place in January this year. The initiative for this course was from the Association of women medical doctors for reproductive medicine and endocrinology (ÄRE), under the organization umbrella of the German society for reproductive medicine (DGRM). The Medical Association of Lower Saxony has certified the course under the Leadership of Prof. Bals-Pratsch of Regensburg. From 12 to 16 January 2011, 6 women gynaecologists specialized in gynaecological endocrinology and reproductive medicine from all over Germany and Austria, gathered to learn and to get an overview of the methods which are used in the laboratories in their own clinical practices. Through the experiences they gathered in this workshop they learned the different methods through demonstration and then performed the manipulations with their own hands. They also became familiar with the problems associated with these manipulations as well as the time and organization required to perform the techniques at consistently high standard. After this experience they will be better able to understand and to manage this aspect of their practices. In Germany, the medical specialist is responsible for the IVF procedure and in general the success of the treatment, so only medical doctors are allowed to perform the in vitro fertilization procedures and the embryo transfers. (Embryonenschutzgesetz 11). Therefore it is extremely important that they as responsible persons, understand all the laboratory techniques.
During the 4 day course, equipment was available for demonstration and use by the participants in a room in the Burghotel Hardenberg, Nörten-Hardenberg. Instructors were available so that the participants could learn and practice oocyte puncture, searching for and finding oocytes, removing cumulus cells from oocytes without damaging them, and sperm preparation. In addition ICSI, laser techniques, and cryo-preservation techniques were demonstrated and practiced by the participants.

All the methods were demonstrated using animal model material. Additionally both fresh and frozen sperm was obtained from the insemination station Gö.
In order to observe semen collection, the participants visited a stallion station in Immensen/Einbeck. They saw the collections, semen processing methods were discussed, and in the laboratory, antral follicles visible on the surface of cow and pig ovaries from the slaughter house were punctured to obtain the cumulus oocyte complexes.
All the participants told us that this area of reproductive medicine was very inspiring, and they all worked very intensively on the techniques. The hands on experiences stimulated many discussions among the participants together with the instructors about the treatment possibilities in the human reproductive medicine.

After the participants had practiced searching for oocytes under stereo microscopes, and the different ways to handle oocytes were tried, the oocytes were therafter available for ICSI training-on the 2 ICSI micromanipulator microscopes. In addition, laser, spindle, and zona imaging as well as blastomere biopsy were demonstrated. These techniques were also attempted by the participants on the cattle and pig oocytes.
A real highlight in the live demonstrations was the harvesting of mouse oocytes from the oviducts. Under camera control, the participants could see how this was done with very fine hand control on the screens, as the technical personel from the Göttinger Max-Planck Institute for Experimental Medicine flushed the gametes and embryos from the oviducts. These were then put in culture medium for later steps in the manipulation.
Over the course 3 evening lectures from; Dr. H. W. Michelmann (Universitätsmedizin Göttingen, Frau Dr. M. Wiarda ("Wertvoller Vertrieb", Hardegsen) und Frau Dr. P. Nayudu (Deutsches Primantenzentrum Göttingen), were presented to stimulate discussion, present research which lay behind the techniques, and to suggest strategies to further improve the clinicial results in this area of medicine.
All the supporting staff who worked with me, the participants, and myself had a lot of fun during the course, working with the techniques, discussing, and getting to know each other. The participants have also obtained many important experiences into the delicacy and complexity of the methods in the IVF laboratory, and are now in a better position to understand and appreciate the skills of their laboratory colleagues.

It is certain, that this course has stimulated intensive contacts and new friendships, and will result in inter-collegial exchanges long after the completion of the course.
I would like here to thank the many people who worked with me in the workshop, the commercial supporters, and the different research units who helped. Not only was the latest technology demonstrated, but also high level know-how and practice relevant seminars, all supported the aims of the workshop.
Most importantly, without the support of the company MSD, represented by Frau Berit Breder, this course would not have been possible. To her and MSD, I give my very special thanks.

Dr. Manuela Ropeter
Dragon IVF International

Success through cooperation.